Despite the numerous actions and functions of the Palmaris longus, its absence and presence is of scientific and medical interest because it varies with sex, laterality, ethnicity, and population. This study investigated the prevalence of the presence and absence of the palmaris longus tendon in the Ikwerre ethnic group of Aluu community of Rivers State, Nigeria. A total of 384 subjects (192 males and 192 females) were recruited for the study. The study made use of the Schaeffer’s test, and confirmation tests by Thompson’s and Pushpakumar’s. Results indicated that 147(76.6%) of females and 160(83.3%) of males exhibited bilateral presence of Palmaris longus tendon (PLT), while unilateral presence was noted in 30(15.6%) of females and 23(12.0%) of males. A total absence of 15(7.8%) and 9(4.7%) in females and males was observed respectively. The differences observed between prevalence of the presence and absence of Palmaris longus and gender, age, and sides (laterality) showed no statistical significant differences (p>0.05). Though no significant difference observed but the differences observed conformed to most researchers’ reports and therefore means that if more sample size were employed for the research probably the differences could have shown significant difference. Therefore the study is recommended to the medical practitioners or scientists concerned to be used for the population understudy.