There are variety of phalangeal creases and broadly classified into various categories and sub-categories. In Anthropology, we studied the variation of creases in same population and different populations. The purpose of the present investigation is to study the bimanual and bisexual variation of medial phalangeal creases among Lodhis of Sagar district (MP), India. The basis of the study was 102 males and 102 females individuals of Lodhi caste. For taking palm prints and for the analysis of phalangeal creases the method prescribed by Bali (1980) is fully followed. The values in this study are of qualitative in nature, so the statistical interpretation of the data has been made by means of the chisquare test. The result shows significant bisexual and bimanual variation of medial phalangeal creases among Lodhis.