The study of Occupational health in petrochemical industry examines to promote health and safety to the workers in India. The Hazards and risk involved in the petrochemical industry is high compared with other industries and least importance is given to worker’s health. Most of accident does not come to the legal formalities. The People are not aware of health and safety is due to the workers are uneducated and management not given importance due to promote Industrial health and Occupational safety in petrochemical industry becomes a barrier in implementing Industrial health and Occupational safety. Present study is an analytical cross sectional, aims to study to determine the effect of petrol fumes on anthropometry and ventilatory function among petrol pump workers in district Pondicherry, Puducherry, India. 84 petrol pump workers with age, sex, weight and height matched controls were recruited from among 102 pump workers in Pondicherry. Using the modified British Medical Research Council Questionnaire, the ventilatory function parameters of the subjects and controls were measured with an microplus spirometer. The mean values of ventilatory function parameters of petrol pump workers were lower than that of controls but only that for PEFR was statistically significant. The mean ventilatory parameters by the work hour per week and by the number of vehicles filled per day were statistically significant for FEV1.0/FVC, PEFR and FEV10, FEV1.0/FVC, PEFR. Abnormal ventilatory function was seen in a good percent of petrol pump workers (29.76%) which consisted of obstructive defects (16.67%) and restrictive defects (13.09%) compared to (13.09%) and (8.33%) in the control groups respectively. The difference was not statistically significant. The mean ventilatory function parameters in workers who smoke was lower than in control groups who smoke. The difference was statistically significant for FEV1.0/FVC. The mean ventilatory function parameters in non-smoking workers were significantly lower than in non-smoking controls. There was no statistically significant difference in the mean ventilatory function parameters of petrol pump workers who smoke and those who do not smoke. The study showed that exposure to petrol fumes among petrol pump workers (who had worked as such for more than one year) had significant effect on their ventilatory function and not much on anthropometry. The study also showed that abnormal ventilatory pattern was prevalent in petrol pump workers, predominant ventilatory dysfunction was obstructive.