Hypothyroidism is defined as the underproduction of the thyroid hormones triiodothyronine (T3) and thyroxine (T4) and an increase in the thyroid stimulating hormone levels. It can lead to the lipid abnormalities which increase the risk of endothelial dysfunction, hypertension and cardiovascular diseases. The data for the present case-control study was collected on 175 females (100 thyroid patients and 75 controls), in the age range of 25 to 55 years from various hospitals and other areas of Mansa district of Punjab. The objectives of the study were to find out the association of the thyroid stimulating hormone with lipid profile and then compare it between cases and controls. The lipid profile (total cholesterol, high density lipoproteins, low density lipoproteins, triglycerides and very low density lipoproteins) and thyroid stimulating hormone analysis was done to evaluate dyslipidemia in hypothyroid patients. It was observed that the mean values for various components of lipid profile and thyroid stimulating hormone were significantly greater in cases as compared to the controls. Further, thyroid stimulating hormone showed a highly significant association with total cholesterol, low density lipoprotein, triglyceride and very low density lipoprotein. It can be concluded that the hypothyroid females were suffering with dyslipidemia, which may be a risk factor for any heart ailment.