The present study was carried out to study the ACE gene polymorphism among the Panika and Baiga of Madhya Pradesh. A total number of 120 individuals (age group 12 to 50) i.e.60 samples each from both the populations constitute the samples for the present study. The highest genotype frequency of ID i.e. 60% is found in both the populations. 20% each of II and DD homozygous genotype were found in Panika whereas 28.33% and 11.66% for II and DD homozygous genotype were found respectively in Baiga. However, both the populations show non-significant difference from each other having p-value of 0.33 (χ2 =2.18) at 95% confidence interval. Hence, it follows Hardy Weinberg Law.