Dopamine receptors play a key role of the mesolimbic dopamine systems in reinforcing effects of alcohol and other drug abuse/addiction. The present study aims to assess the association of DRD1 gene polymorphism (-48 A/G DdeI) with alcohol dependence (AD). A case-controls study was carried out comparing 143 AD cases, who all met DSM-IV criteria for AD, and 318 age sex match controls from Meiteis of Manipur, India. Smoking status were SIGNIFICANTLY associated with AD (OR=2.983, 95% CI 1.970-4.517, p=0.000). Genotypic and allelic frequency distribution of -48A/G DdeI polymorphism did not differ significantly between the two groups. However, in recessive model mutant homozygous (GG) has shown an OR of 1.03, though not significant (95% CI=0.583-1.820; p=0.92). Interaction analysis of DRD1 -48A/G DdeI polymorphism and smoking status showed borderline significant increase risk for AD (OR=1.463, 95% CI 0.993-2.155, p=0.054). The present study suggests that -48A/G DdeI gene polymorphism is not a risk genetic marker for AD, though risk is likely to be increase among individuals possessing G allele and who smoked in the studied population.