Researchers have inclined their focus for better perception of mechanism and efficacy of medicinal plants in neurodegenerative disorders. Bacopa monnieri (BM) has been the fascinating candidate to treat the disorders of mental frailty. Cerebral disorders are the upshots of disparity between cellular oxidant and antioxidant levels. Hydrogen peroxide (H2O2), pioneer of various detrimental reactive oxygen species (ROS), induces oxidative damage ensuing brain infirmity. The current study was designed to investigate the neuroprotective potency of BM extracts against H2O2 induced oxidative damage in C6 glioma cells. The protective efficacy of methanolic, ethanolic and water extracts of BM (BM-MEx, BM-EEx and BM-WEx) was determined by MTT assay. The prior treatment of BM-MEx
remarkably upregulated the expression levels of antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase and glutathione in the H2O2 exposed cells. The lipid peroxidation level was found to be remarkably down-regulated with BM-MEx pre -treatment. Immunofluorescence and RT-PCR demonstrated that the prior treatment of BM-MEx significantly alleviated the expression of GFAP (cytoskeletal marker), HSP70 and Grp75 (stress markers) in H2O2 exposed cells. The current investigation suggests that BM-MEx can
provide protective ability to combat against cerebral infirmity.